FIGURE 3.

(A) Sensitivity comparison results of Zaire ebolavirus. The real-time RT-PCRs were amplified by the 7,500 Real-Time PCR System (ABI, Foster City, CA, United States). Five dilutions (ranging from 10⁵ to 10¹ copies/μl) of Zaire ebolavirus in vitro-transcribed RNA templates were amplified to compare the sensitivity of our microarray with that of the Ebola virus (Zaire) Nucleic Acid Detection Kit (real-time PCR) (Shenzhen Puruikang Biotech Co., Ltd.). (B) Relationship between microarray signals and PCR cycles. Real-time RT-PCR and microarray of 16 pathogens were performed simultaneously, and the diagram display the relationship between microarray signals and PCR cycles obtained.