TABLE 1.
The primers and their concentrations in multiplex PCR.
| Group ID | Primersa | Sequence (5′–3′)b | Primer conc. in multiplex PCR (mM) | Targeted genes | Pathogens | GenBank accession no. |
| A tube | EBO-F | TCTTGAAATMAARAAACCTGAC | 0.14 | GP | ZEBOV and SEBOV | KM233101 |
| EBO-R | ACCGGGGRAAGCCYCGAATC | 0.7 | KC589025 | |||
| MAR-F | AATAAGAAAGTGATATTATTTGACACAA | 0.1 | NP | MARV | Z12132 | |
| MAR-R | TGTTGAATTTATCCTTATCAGAATT | 0.5 | ||||
| LF-F | TCAAGGACTTCAATAACA | 0.1 | NP | LFV | AY628201 | |
| LF-R | CCCTGCAGACTGCAAGGATTTG | 0.5 | ||||
| JUN-F | CCATCAGGTTATGTTAAGG | 0.1 | L | JUNV | JN801477 | |
| JUN-R | CTATGGTGGTGGTGCTG | 0.5 | ||||
| MAC-F | ATATGAAGGGAGGTGTGAACA | 0.1 | GP1 | MACV | AY571930 | |
| MAC-R | GCTTGAGTCAGACTTATTGAGACA | 0.5 | ||||
| B tube | RVF-F | GGAGAATTCCCATACCGAGTCG | 0.08 | NS | RVFV | KF648860 |
| RVF-R | GTGAAATCACTGAGAGTCATATGG | 0.4 | ||||
| CCHFV-F1 | TCTCAAAGAAACACGTGC | 0.1 | NP | CCHFV | AF362080 | |
| CCHFV-R1 | CACAAGTCCATTTCCTTTCTTGAAC | 0.5 | ||||
| MAL-F1 | GTAATCTTAACCATAAACTATGC | 0.1 | 18s rRNA | MP | HQ283215 | |
| MAL-R1 | TGTCAATCCTACTCTTGTCTTA | 0.5 | ||||
| HV-MFO | AAAAGTAGGTGITAYATCYTIACAATGTGG | 0.16 | M | HV | AF288298 | |
| HV-MRO | GTACAICCTGTRCCIACCCC | 0.8 | ||||
| SFTSV-F | AGCATGAATTCTCACGGAGC | 0.12 | L | SFTSV | KP202163 | |
| SFTSV-R | CGCTCTTCAAGGTTCTGCTT | 0.6 | ||||
| C tube | DEN-F | TCCACCTGAGAAGGTGTAARAAATCCG | 0.1 | 3′UTR | DENV | GQ398257 |
| YFV-F1 | GACTCCACACATTGAATAGA | 0.1 | 3′UTR | YFV | U54798 | |
| FLA-Rc | ATGTCTCCTCTACCCTCTAGWACT | 0.5 | 3′UTR | Flavivirus | GQ398257 | |
| CHIKV-F1 | GATCATAGATGCAGTTGTAT | 0.1 | NSP4 | CHIKV | DQ443544 | |
| CHIKV-R2 | CGCCGTACAAAGTTATGACG | 0.5 | ||||
| FA-MF2 | GGCCCCCTCAAAGCCGAGAT | 0.1 | M | Flu A | HQ664927 | |
| FA-MR2 | CAAAGCGTCTACGCTGCAGT | 0.5 | ||||
| FB-F1 | ATGGCCATCGGATCCTCAACTCACTC | 0.1 | NS | Flu B | CY099917 | |
| FB-R1 | TCATGTCAGCTATTATGGAGCTGTT | 0.5 |
aF, forward primers; R, reverse primers.
bAll the reverse primers with a biotin-labeled 5′-end.
cConsensus reverse primer for DENV and YFV.
ZEBOV, Zaire ebolavirus; SEBOV, Sudan ebolavirus; MARV, Marburg virus; LFV, Lassa fever virus; JUNV, Junin virus; MACV, Machupo virus; RVFV, Rift Valley fever virus;
CCHFV, Crimean-Congo hemorrhagic fever virus; MP, malaria parasite; HV, hantavirus; SFTSV, severe fever with thrombocytopenia syndrome virus; DENV, Dengue virus,
YFV, yellow fever virus; CHIKV, Chikungunya virus, FluA, influenza A virus; FluB, influenza B virus; JEV, Japanese encephalitis virus; HPIV, human parainfluenza virus.