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. 2021 Jul 30;15:699329. doi: 10.3389/fnins.2021.699329

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Copyright © 2021 Chesworth, Gamage, Ullah, Sonego, Millington, Fernandez, Liang, Karl, Münch, Niedermayer and Gyengesi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative confocal images of reconstructed microglia from WT standard diet-fed (A,B), GFAP-IL6 standard diet-fed (C,D), WT apigenin-fed (E,F), and GFAP-IL6 apigenin-fed (G,H) cohorts. The extended depth of focus images obtained from collapsing 3D confocal microscopy images of Iba-1⁺ microglia obtained under 63× oil immersion objective, along with the corresponding manually reconstructed images in Neurolucida 360. GFAP-IL6 mice (apigenin diet) (G,H) had a significantly reduced soma area, soma perimeter and a larger number of nodes than that of GFAP-IL6 mice (standard diet) (C,D). (Scale bar 10 μm).