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. Author manuscript; available in PMC: 2021 Aug 13.
Published in final edited form as: Alzheimers Dement. 2018 Jul 4;14(10):1302–1312. doi: 10.1016/j.jalz.2018.05.017

Fig. 3.

Fig. 3.

Knockdown of the NR1 subunit of NMDAR prevents AβO-induced neuronal CCR. (A) Primary cortical neurons were transduced for 96 hours prior to AβO addition with lentivirus expressing shRNA to NR1, or as a control, with lentivirus comprising an empty expression vector. After 16–18 hours of AβO exposure, the cells were stained by triple immunofluorescence for NeuN and MAP2 to mark neurons, and for cyclin D1 to assess CCR. (B) Quantification of the immunofluorescence results. Quantification of the immunofluorescence results and statistical analysis by one-way ANOVA. Error bars indicate s.e.m. (C) Quantitative western blot showing a 30% knockdown of NR1. Abbreviations: ANOVA, analysis of variance; AβO, amyloid-β oligomer; CCR, cell cycle reentry; NMDAR, N-methyl-D-aspartate receptor.