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. 2021 Jul 30;12:674532. doi: 10.3389/fimmu.2021.674532

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Copyright © 2021 Fernandes, Patil, Meriaux, Theresine, Muller, Leenen, Elwenspoek, Zimmer and Turner

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Unbiased immunophenotyping with viSNE. (A) visNE map obtained through cytobank with the markers a single 13-color antibody combination. Each panel represents the mean of all 12 animals in the control (left), MD₁₅ (middle) and MD₃₀ (right) groups; (B) Identification of different clusters by the principal rat cell surface markers – CD3 CD4, CD8, CD11b/c, CD45RA, FoxP3. Stars represent significantly different clusters, between the different treatment groups at an FDR corrected p < 0.05; (C) Clusters colored by CD161a (NK cell marker) intensity. Blue to orange: low to high expression of the marker.