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. 2021 Jul 30;12:674532. doi: 10.3389/fimmu.2021.674532

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Copyright © 2021 Fernandes, Patil, Meriaux, Theresine, Muller, Leenen, Elwenspoek, Zimmer and Turner

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Maternal deprivation induces long-term changes in the immune system. (A) Adaptive immune cell population analysis. T cells gated as CD3+ from total lymphocytes. B cells gated as CD45RA+ gated from total lymphocytes; CD4+ and CD8+ gated from within the T cell population (B) expression levels of NK and NKT-like cells. NK and NKT cells were gated from total lymphocytes as the brighter population expressing CD161a+ and being CD3- or CD3+, respectively.; (C) Sub-gating of the NKT-cell population from panel (B) by CD4⁺ and CD8⁺ markers. Data is presented as mean ± SEM of 12 animals per group. Statistics: Two-way ANOVA; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001, NS, Not Significant. Representative Figures for flow data are included in the Supplementary Material.