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. 2021 Jul 30;12:674532. doi: 10.3389/fimmu.2021.674532

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Copyright © 2021 Fernandes, Patil, Meriaux, Theresine, Muller, Leenen, Elwenspoek, Zimmer and Turner

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Maternal deprivation changes the degranulation capacity of the NK cells from ELA individuals. Cells were gated as lymphocytes from FSC-H, SSC-H; singlets from SSC-H, SSC-A; then from CD3-CD19- cells. (A) representative image of the NK cell population gating strategy: 1– CD56^brightCD16^-; 2 – CD56^brightCD16⁺; 3- CD56^dimCD16⁺; 4– CD56^dimCD16^dim; 5- CD56^dimCD16^bright; 6 – CD56^-CD16^bright; (B, C) expression of CD107a by populations 5 and 6; (D–F) expression of IFN-γ by populations 4, 5 and 6. Data is presented as mean ± SEM of 14 donors per group. Statistics: Two-way ANOVA; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.