Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Drosophila melanogaster) | Antp | Flybase:FB2020_01 | FLYB:FBgn0260642 | |
| Gene (Drosophila melanogaster) | Hml | Flybase:FB2020_01 | FLYB:FBgn 0029167 |
|
| Gene (Drosophila melanogaster) | Collier/kn | Flybase:FB2020_01 | FLYB:FBgn0001319 | |
| Gene (Drosophila melanogaster) | wg | Flybase:FB2020_01 | FLYB: FBgn0284084 |
|
| Gene (Drosophila melanogaster) | hep | Flybase:FB2020_01 | FLYB:FBgn0010303 | |
| Gene (Drosophila melanogaster) | EcR | Flybase:FB2020_01 | FLYB:FBgn0000546 | |
| Gene (Drosophila melanogaster) | PGRP-LB | Flybase:FB2020_01 | FLYB:FBgn0037906 | |
| Gene (Drosophila melanogaster) | Tak1 | Flybase:FB2020_01 | FLYB:FBgn0026323 | |
| Gene (Drosophila melanogaster) | bsk | Flybase:FB2020_01 | FLYB:FBgn 0000229 |
|
| Gene (Drosophila melanogaster) | Ena | Flybase:FB2020_01 | FBgn0000578 | |
| Gene (Drosophila melanogaster) | Hh | Flybase:FB2020_01 | FBgn0004644 | |
| Gene (Drosophila melanogaster) | Dia | Flybase:FB2020_01 | FBgn0011202 | |
| Genetic reagent (D. melanogaster) | Antp-Gal4 | Emerald and Cohen, 2004 | FLYB:FBal0155891 | FlyBase symbol: GAL4Antp-21 |
| Genetic reagent (D. melanogaster) | P(col5-cDNA)/CyO-TM6B, Tb | Krzemień et al., 2007 | FLYB:FBti0077825 | FlyBase symbol: P{GAL4}col85 |
| Genetic reagent (D. melanogaster) | Hml-GAL4.Δ | Sinenko and Mathey-Prevot, 2004 | FLYB:FBtp0040877 | FlyBase symbol:P{Hml-GAL4.Δ} |
| Genetic reagent (D. melanogaster) | UAS-Rel RNAiKK | Vienna Drosophila Resource Center | VDRC:v108469; FLYB:FBti0116709; RRID:FlyBase_FBst0477227 |
FlyBase symbol: P{KK100935}VIE-260B |
| Genetic reagent (D. melanogaster) | w[1118] | Bloomington Drosophila Stock Center | BDSC:3605; FLYB:FBal0018186;RRID:BDSC_3605 | FlyBase symbol: w1118 |
| Genetic reagent (D. melanogaster) | UAS-Rel RNAi | Bloomington Drosophila Stock Center | BDSC:33661; FLYB:FBti0140134;RRID:BDSC33661 | FlyBase symbol: P{TRiP.HMS00070}attP |
| Genetic reagent (D. melanogaster) | UAS-wg RNAi | Bloomington Drosophila Stock Center | BDSC:33902; FLYB:FBal0263076; RRID:BDSC_33902 | FlyBase symbol: P{TRiP.HMS00844}attP2 |
| Genetic reagent (D. melanogaster) | UAS-dia RNAi | Bloomington Drosophila Stock Center | BDSC:35479; FLYB:FBtp0068562; RRID:BDSC_35479 |
FlyBase symbol: P{TRiP.GL00408} |
| Genetic reagent (D. melanogaster) | UAS-hep.Act | Bloomington Drosophila Stock Center | BDSC:9305; FLYB:FBti0074410; RRID:BDSC_9305 | FlyBase symbol: P{UAS-Hep.Act}1 |
| Genetic reagent (D. melanogaster) | UAS-FUCCI | Bloomington Drosophila Stock Center | BDSC:55121; RRID:BDSC_55121 | FlyBase symbol: P{UAS-GFP.E2f1.1–230}32; P{UAS-mRFP1.NLS.CycB.1–266}19 |
| Genetic reagent (D. melanogaster) | TRE-GFP | Bloomington Drosophila Stock Center | BDSC:59010; FLYB:FBti0147634; RRID:BDSC_59010 | FlyBase symbol: P{TRE-EGFP}attP16 |
| Genetic reagent (D. melanogaster) | Pxn-YFP | Kyoto Stock Center | kyoto:115452; FLYB: FBti0143571; RRID:FlyBase_FBst0325439 |
FlyBase symbol: PBac{802 .P.SVS-2}PxnCPTI003897 |
| Genetic reagent (D. melanogaster) | hhF4f-GFP | Tokusumi et al., 2012 | FBtp0070210 | FlyBase symbol:P{hhF4f-GFP} |
| Genetic reagent (D. melanogaster) | UAS-GMA | Bloomington Drosophila Stock Center | BDSC:31774; FLYB:FBti0131130; RRID:BDSC_31774 | FlyBase symbol:P{UAS-GMA}1 |
| Genetic reagent (D. melanogaster) | UAS-Rel 68kD | Bloomington Drosophila Stock Center | BDSC:55778; FLYB:FBti0160486; RRID:BDSC_55778 | FlyBase symbol: P{UAS-FLAG-Rel.68}i21-B |
| Genetic reagent (D. melanogaster) | UAS-Rel 68kD | Bloomington Drosophila Stock Center | BDSC:55777; FLYB:FBti0160484 RRID:BDSC_55777 |
FlyBase symbol: P{UAS-FLAG-Rel.68} |
| Genetic reagent (D. melanogaster) | UAS-EcR.B1Δ | Bloomington Drosophila Stock Center | BDSC:6872; FLYB:FBti0026963; RRID:BDSC_6872 | FlyBase symbol: P{UAS-EcR.B1-ΔC655.W650A}TP1-9 |
| Genetic reagent (D. melanogaster) | PGRP-LB[Delta] | Bloomington Drosophila Stock Center | BDSC:55715; FLYB:FBti0180381; RRID:BDSC_55715 | FlyBase symbol: TI{TI}PGRP-LBΔ |
| Genetic reagent (D. melanogaster) | wgl-12 cn1 bw1/CyO | Bloomington Drosophila Stock Center | BDSC:7000; FLYB:FBal0018504; RRID:BDSC_7000 | FlyBase symbol: wgl-12 |
| Genetic reagent (D. melanogaster) | Tak1(2) | Bloomington Drosophila Stock Center | BDSC:26272; FLYB:FBal0131420; RRID:BDSC_26272 | FlyBase symbol: dTak12 |
| Gene (Drosophila melanogaster) | RelE20 | Flybase:FB2020_01 | FLYB:FBgn0014018 | |
| Genetic reagent (D. melanogaster) | UAS-bsk[DN] | Bloomington Drosophila Stock Center | BDSC:6409; FLYB:FBti0021048; RRID:BDSC_6409 | FlyBase symbol: P{UAS-bsk.DN}2 |
| Genetic reagent (D. melanogaster) | UAS-ena RNAiKK | Vienna Drosophila Resource Center | VDRC: v106484 FBst0478308; RRID:v106484 |
FlyBase symbol: P{KK107752}VIE-260B |
| Genetic reagent (D. melanogaster) | UAS-mCD8: RFP | Bloomington Drosophila Stock Center | BDSC:27400; FLYB:FBti0115747; RRID:BDSC_27400 | FlyBase symbol: P{UAS-mCD8.mRFP.LG}28a |
| Genetic reagent (D. melanogaster) | tubGAL80[ts20] | Bloomington Drosophila Stock Center | BDSC:7109; FLYB:FBti0027796; RRID:BDSC_7109 | FlyBase symbol: P{tubP-GAL80ts}20 |
| Antibody | Anti-P1 (Mouse monoclonal) | Kurucz et al., 2007 | Cat# NimC1, RRID:AB_2568423 | IF(1:50) |
| Antibody | Anti-c Rel (Mouse monoclonal) | Stöven et al., 2000 | Cat#21F3, RRID:AB_1552772 |
IF (1:50) |
| Antibody | Anti-Ci155 (Rat polyclonal) | Developmental Studies Hybridoma Bank | Cat# 2A1, RRID:AB_2109711 |
IF(1:2) |
| Antibody | Anti-Wg (Mouse monoclonal) | Developmental Studies Hybridoma Bank | Cat#4D4 RRID:AB_528512 |
IF(1:3) |
| Antibody | Anti-Singed (Mouse monoclonal) |
Developmental Studies Hybridoma Bank | Cat# sn 7C RRID:AB_528239 |
IF(1:20) |
| Antibody | Anti-Enabled (Mouse monoclonal) |
Developmental Studies Hybridoma Bank | Cat#5G2 RRID:AB_528220 |
IF(1:30) |
| Antibody | Anti-PH3(Rabbit monoclonal) | Cell signaling Technology |
Cat# 3642S RRID:AB_10694226 |
IF(1:150) |
| Antibody | Anti-Hh (Rabbit monoclonal) | Forbes et al., 1993 | IF(1:500) | |
| Antibody | Anti-Hnt (Mouse monoclonal) |
Developmental Studies Hybridoma Bank | Cat#1G9 RRID:AB_528278 |
IF(1:5) |
| Antibody | Anti-EcR common (Mouse monoclonal) |
Developmental Studies Hybridoma Bank | Cat#DDA2.7 RRID:AB_10683834 |
IF(1:20) |
| Antibody | Anti-Ance (rabbit monoclonal) | Hurst et al., 2003 | IF(1:500) | |
| Antibody | Anti-GFP (rabbit polyclonal) |
Cell signaling Technology |
Cat#2555 | IF(1:100) |
| Antibody | Anti-shg (rat monoclonal) |
Developmental Studies Hybridoma Bank | Cat#DCAD2 RRID:AB_528120 |
IF(1:50) |
| Antibody | Anti-β-PS (mouse monoclonal) |
Developmental Studies Hybridoma Bank | Cat#CF.6G11 RRID:AB_528310 |
IF(1:3) |
| Antibody | Anti-DIG-POD (sheep polyclonal) | Sigma-Aldrich | Cat#11207733910 | IF(1:1000) |
| Chemical compound, drug | Phalloidin from Amanita phalloides | Sigma-Aldrich | Cat#P2141 | IF(1:500) |
| Chemical compound, drug | Rhodamine Phalloidin | Thermo Scientific | Cat# R415 RRID:AB_2572408 |
IF(1:500) |
| Sequence-based reagent | Relish cDNA clone | DGRC | Clone id: GH01881 FLYB: FBcl0110737 |
|
| Sequence-based reagent | Actin_F | Elgart et al., 2016 | PCR primers | GGAAACCACGCAAATTCTCAGT |
| Sequence-based reagent | Actin_R | Elgart et al., 2016 | PCR primers | CGACAACCAGAGCAGCAACTT |
| sequence-based reagent | Aceto_F | Elgart et al., 2016 | PCR primers | TAGTGGCGGACGGGTGAGTA |
| Sequence-based reagent | Aceto_R | Elgart et al., 2016 | PCR primers | AATCAAACGCAGGCTCCTCC |
| Sequence-based reagent | Lacto_F | Elgart et al., 2016 | PCR primers | AGGTAACGGCTCACCATGGC |
| Sequence-based reagent | Lacto_R | Elgart et al., 2016 | PCR primers | ATTCCCTACTGCTGCCTCCC |
| Software, algorithm | Fiji | Fiji | RRID:SCR_002285 | |
| Software, algorithm | Photoshop CC | Adobe | RRID:SCR_014199 | |
| Software, algorithm | Imaris | Bitplane | RRID:SCR_007370 | |
| Commercial assay or kit | Click-iTEdU plus (DNA replication kit) | Invitrogen | Cat# C10639 | |
| Commercial assay or kit | Alexa Fluor 594 Tyramide Reagent | Thermo Fischer | Cat# B40957 |