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. 2021 Aug 16;131(16):e150398. doi: 10.1172/JCI150398

Figure 1. CF ASL is abnormally acidic at baseline but alkalinizes with TNF-α+IL-17.

Figure 1

Primary cultures of differentiated human airway epithelia were derived from multiple donors. pHASL was measured using SNARF-1-conjugated dextran on an inverted confocal microscope. (A) Non-CF (blue) versus CF (red) pHASL at baseline and after treatment with TNF-α (10 ng/ml) and IL-17 (20 ng/ml) for 48 hours (n = 6–9). (B) CF epithelia treated with TNF-α+IL-17 for 24 hours. pHASL was measured in the presence of HCO3–/CO2 as well as after removing CO2 from the environment and replacing HCO3– with HEPES (n = 6). Each data point represents an epithelium from a different donor. Data are shown as the mean ± SEM. Statistical significance was tested using ANOVA with post test Tukey’s. *P < 0.05, **P < 0.01, ****P < 0.0001.