Figure 5. Prognostic significance of the smoking-associated DNA methylation signature.

(A) Kaplan-Meier analysis for relapse-free survival (RFS) prediction by MIND in the 26 CAF samples. Patients were stratified by the MIND^high and MIND^low groups without reliance on survival data (P = 0.013, log-rank test). (B) Significance of MIND in recurrence prediction in the validation cohort of 14 CAF samples (P = 0.007, log-rank test). Patients were stratified into the MIND^high and MIND^low groups using the median as cutoff. (C and D) Significance of MIND for its prognostic power by applying to DNA methylation profiling of tumor samples from 2 independent cohorts, GSE39279 and TCGA-LUAD. (C) The 431 NCSLC patients in GSE39279 were split evenly into the MIND^high and MIND^low groups using the median as cutoff. After removing 241 patients with missing survival data, the log-rank test was performed on the remaining 190 patients (P = 0.003). (D) The 449 patients in the TCGA-LUAD cohort were split evenly into the MIND^high and MIND^low groups using the median as cutoff. After removing 9 patients with missing survival data, the log-rank test was performed on the remaining 440 patients (P = 0.018). OS, overall survival.