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. 2021 Aug 16;131(16):e148979. doi: 10.1172/JCI148979

Figure 6. Ex vivo–based bulk CD8+ T cell activation by HIV-1–infected target cells expressing HLA-E and CD4.

Figure 6

221ΔE cell lines expressing CD4 and either HLA-E*01:01, HLA-E*03:01, or HLA-B*57:01 were infected with NL4-3 virus (MOI = 0.5) for 12 hours, cultured for 48 hours, then cocultured with freshly isolated bulk CD8+ T cells from HIV-infected individuals (n = 7). CD8+ T cell production of IFN-γ alone or CD107a in combination with granzyme B, perforin, or IFN-γ was assessed after 12 hours of coculture with APCs. (A and B) HLA-E*01:01–, HLA-E*03:01–, and HLA-B*57:01–restricted CD8+ T cell responses from 2 representative individuals (patient 5 and patient 9; Supplemental Table 4). Red boxes indicate positive responses compared with uninfected controls. (C) Cumulative data from 7 individuals with both HLA-E*01:01– and HLA-B*57:01–restricted responses. Median values are shown, and error bars represent interquartile range. Data within groups were compared using Wilcoxon signed rank test.