Figure 7. JQ1 treatment of dysfunctional CAR T cells from patients with CLL is associated with a unique transcriptional profile.

(A) Volcano plot showing differentially expressed genes identified through RNA-Seq of relapsed/refractory CLL patient CAR⁺CD8⁺ T cells treated with (–)-JQ1 or (+)-JQ1 for 4 days (n = 4). Downregulated genes are colored in blue and upregulated genes are depicted in red. Unadjusted P values are shown. (B) Gene ontology terms associated with genes that were significantly upregulated (red) and downregulated in the above JQ1-treated patient CD8⁺CAR⁺ T cells. (C) Representative GSEA results from running the unfiltered CD8⁺CAR⁺ T cell (+)-JQ1 versus (–)-JQ1 rank list against the Molecular Signatures Database (MSigDB) gene ontology collections.