Figure 3. CHI3L1 induces M2-like MDM accumulation in vivo.

Representative flow cytometry analyses and quantitation showing the percentages of M1- and M2-like MDMs in tumors derived from GL261 (A and B) and QPP7 (C and D) glioma-bearing mice with altering CHI3L1 expression. (E) Ratio of CD206^–:CD206⁺ cells from the CD45⁺CD68⁺CD11b⁺ cell population and ratio of iNOS⁺:Arg1⁺ cells from the CD45⁺ cell population in QPP7-derived tumors. Each dot represents 1 mouse; data are presented as mean ± SEM. P values were calculated using 1-tailed, unpaired t test. (F) Representative IF images for F4/80⁺ and P2Y12⁺ cells in tumor sections from the syngeneic mice bearing GL261-CHI3L1 versus vector control. (G) Quantitation of indicated cells in peritumoral and intratumoral regions, respectively. Representative IF images and quantitation for F4/80⁺ (H) and P2Y12⁺ (I) cells in tumor sections from QPP7 glioma-bearing mice with shChi3l1#2 versus shSC. Peritumoral and intratumoral regions are separated using yellow lines. Each dot represents 1 field of the peritumoral or intratumoral region from indicated tumors (n ≥ 3). Data are presented as mean ± SD. P values were calculated using 1-tailed, unpaired t test. Scale bars: 100 μm.