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. 2021 Aug 16;131(16):e147552. doi: 10.1172/JCI147552

Figure 4. CHI3L1 induces cell migration of M2-like MDM in vitro.

Figure 4

(A) Representative brightfield images showing cell migration at 0 and 6 hours after treatment with CHI3L1 recombinant protein (rCHI3L1) at a concentration of 0.6 μg/mL in M0, M1, and M2 BMDMs by the scratch-wound healing assay. (B) Cell migration was assessed by quantifying occupied areas by migrated cells. (C) Representative brightfield images for cell migration of M2 BMDMs by the Transwell assay. Migration was assessed by determining the number of migrated cells. Data are presented as mean ± SD from at least 3 independent experiments. P values were calculated using 1-tailed, unpaired t test. ***P < 0.001; ****P < 0.0001. (D) Association between CHI3L1 mRNA expression and M1/M2-like macrophage scores in IDH-WT GBM. Gene expression was normalized by RMA, and P values were calculated by Spearman’s rank correlation.