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. 2021 Aug 16;131(16):e147552. doi: 10.1172/JCI147552

Figure 6. Gal3BP competes with Gal3 for binding with CHI3L1.

Figure 6

(A) A binding model of N-terminal Gal3 (magenta Asp3-Pro17 from PDB 6FOF) and CHI3L1 (green surface with red/blue/white shades corresponding to O/N/H atoms from PDB 1HJV_A). (B) Detailed view from A of the binding mode of Asp3-Asn16 of Gal3 (yellow) and CHI3L1 (green). (C) Representative IF images showing colocalization of proteins in TS603 cells. Scale bar: 20 μm. (D) IB analysis of protein-binding complexes using co-IP with Gal3 antibody in TS603-V5-CHI3L1 cells treated with DMSO or TD139 (10 μM for 24 hours). (E) IB analysis of Gal3 and CHI3L1 protein binding in the mixture of rGal3 and CHI3L1 (200 ng rGal3 + 200 ng rCHI3L1) by adding different amounts of rGal3BP (0, 100, 200, 400, 800 ng/sample) with or without TD139 (10 μM for 1 hour).