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. 2021 Aug 16;131(16):e147268. doi: 10.1172/JCI147268

Figure 2. The impact of 2-DG on the intracellular milieu of BMDMs.

Figure 2

(A) XTT assay from infected BMDMs treated with 5 mM 2-DG or 200 μM apocynin 24 hours after infection. One-way ANOVA with Tukey’s multiple-comparison test. (B) Intracellular pH measurement of infected BMDMs after 6 hours of 2-DG treatment (5 mM). Two-tailed t test. (C) XTT (survival) assay from infected BMDMs, MOI 1:5, treated with 5 mM 2-DG6P. Inside of BMDMs, after 6 hours of infection, yeasts were recovered from BMDMs and treated with 5 mM 2-DG6P for 24 hours; subsequently, XTT assays were performed. Two-way ANOVA with Sidak’s multiple-comparison test. Data are representative of 4 (A), 5 (B), or 6 (C) independent experiments. Violin plots show the median (line) and quartiles (dashed line). ****P < 0.0001.