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. 2021 Aug 16;131(16):e147268. doi: 10.1172/JCI147268

Figure 7. Zinc deficit results in H.

Figure 7

capsulatumdeath. (A) XTT assay from infected BMDMs treated with 2-DG with or without metal supplementation for 24 hours. One-way ANOVA with Tukey’s multiple-comparison test. (B) XTT assay from BMDMs infected with metal-loaded H. capsulatum treated with 2-DG for 24 hours. Two-tailed t test. (C) XTT assay from BMDMs lacking MT1/2 and MT3 treated with 2-DG for 24 hours. One-way ANOVA with Tukey’s multiple-comparison test. (D) Gene expression of zinc transporters from H. capsulatum within macrophages treated with 2-DG for 6 hours. Two-way ANOVA with Sidak’s multiple-comparison test. The housekeeping gene and all data were normalized to H. capsulatum–only as reference (dotted line). (E) XTT from H. capsulatum treated with 5 mM 2-DG, 5 mM 2-DG6P with zinc-deprived media, and in different pH. One-way ANOVA with Tukey’s multiple-comparison test. BMDMs were infected with MOI 1:5 treated with 5 mM 2-DG (A–D). Data are representative of 4 (A, C, and D), 8 (B), or 6 (E) independent experiments. Violin plots show the median (line) and quartiles (dashed line). *P < 0.05; ***P < 0.001; ****P < 0.0001.