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. 2021 Aug 16;131(16):e141083. doi: 10.1172/JCI141083

Figure 4. Slit2 promotes TAM recruitment and polarization in mouse gliomas.

Figure 4

(A) Immunohistochemistry on sections of late-stage CT-2A shCTRL, shSlit2, or shSlit2 + hSLIT2 tumors for F4/80, MHC-II, and MRC1+ cells (green). (B) Quantifications of A (n = 7 mice per group, 5 fields per tumor, 2-way ANOVA). (C and D) FACS analysis of day 21 CT-2A shCTRL and shSlit2 tumors for quantification of TAMs (n = 10 tumors/group; Student’s t test and 2-way ANOVA). (E) qPCR analysis from FACS-sorted TAMs (n = 6 tumors/group, Mann-Whitney U test). (F–H) ELISA from protein samples extracted from FACS-sorted TAMs from shCTRL and shSlit2 tumors to quantify IFN-γ (F), IL-10 (G), and VEGFa (H) (n = 5 tumors/group, Mann-Whitney U test). (I) Representative images and quantification of soluble-Flt1 binding to sections of day 21 CT-2A shCTRL, shSlit2, and day 18 shSlit2 + hSLIT2 tumors (n = 7 mice per group, 5 fields per tumor, 1-way ANOVA). *P < 0.05, **P < 0.01, ***P < 0.001.