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. 2021 Aug 16;131(16):e141083. doi: 10.1172/JCI141083

Figure 6. Slit2 drives microglia and macrophage migration and tumor supportive polarization.

Figure 6

(A–C) Transwell assay of microglial cells (A), BMDMs (B), and PMs (C) in response to Slit2 or carrier (CTRL) in the bottom chamber (n = 4, 1-way ANOVA). (D and E) Transwell assay of RAW macrophages treated or not with Robo1/2 siRNA and infected with adenovirus encoding CTRL (GFP construct), Robo1FL, or Robo1ΔCD constructs and stained with calcein. (E) Quantification of D (n = 3, 2-way ANOVA). (F) Western blot analysis of Slit2 downstream signaling in cultured BMDMs (n = 6). (G and H) ELISA from conditioned medium from LPS-, Il-10–, or Slit2-treated BMDMs to quantify IL-10 (G) and VEGFa (H) (n = 3 independent cultures, Mann-Whitney U test). (I) qPCR analysis of BMDM cultures following Slit2 or LPS treatment (n = 4, 1-way ANOVA or Mann-Whitney U test). Data are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.