Table 1.
Protein | Peptide | Light precursor (m/z) | Precursor charge | Heavy precursor (m/z) | Fragments monitored | Used for quantitation |
---|---|---|---|---|---|---|
CXCL10 | VEIIATMK | 452.7622 | 2 | 456.7693 | y4, y5, y6 | x |
MX1 | DVPDLTLIDLPGITR | 819.4591 | 2 | 824.4632 | y7, y8, y9, y10 | x |
IFFENHPYFR | 457.2278 | 3 | 460.5639 | y3, y4, y5, y6 | ||
ISG15 | IGVHAFQQR | 528.2909 | 2 | 533.295 | y4, y5, y6, y8 | x |
LTQTVAHLK | 505.8033 | 2 | 509.8104 | y5, y6, y7, y8 | ||
STAT1 | VMAAENIPENPLK | 853.9649 | 2 | 717.3811 | y7, y8, y9, y10, y11 | x |
LLGPNASPDGLIPWTR | 713.374 | 2 | 858.969 | y7, y9, y10, y11 | ||
DDX58 | VVFFANQIPVYEQQK | 905.4803 | 2 | 909.4874 | y7, y8, y10, y11 | x |
TNQNTGMTLPAQK | 702.351 | 2 | 706.3581 | y4, y8, y9, y10 |
Targeted proteins and corresponding peptides are listed in each row. Parameters such as precursor ion m/z, charge, and fragment ions monitored are reported for each peptide. Stable isotope–labeled amino acids for each internal standard peptide are shown colored in red. Peptides from each protein that were used for relative quantitation between groups are indicated.