Figure 3.

ROS scavenger treatment attenuated PrP (106-126)-mediated mitochondrial ROS and neurotoxicity. SK-N-SH cells were pretreated with NAC (N-acetyl-l-cysteine) (1 h) at 2.5 and 5 mM and then exposed to 100 μM PrP (106-126) for 6 hours. (a) Cell viability was evaluated by an annexin V assay using FITC-annexin V, which combines with phosphatidylserine on the plasma membrane during the apoptotic processes. (b) Bar graph showing the averages of the annexin V-negative cells. Values represent the mean ± SEM (n = 10). ^∗∗∗p < 0.001 vs. PrP. (c) Mitochondrial ROS was evaluated by a MitoSOX assay. (d) Bar graph showing the averages of the red fluorescence (MitoSOX). Values represent the mean ± SEM (n = 10). ^∗∗p < 0.01 vs. PrP.