Figure 4.

PrP (106-126) did not influence SOD or catalase enzyme. SK-N-SH cells were pretreated with DDC (diethyldithiocarbamate) (1 h) or AT (3-amino-1,2,4-triazole) (24 h) and then exposed to 100 μM PrP (106-126) for 6 hours. (a) Mitochondrial ROS was evaluated by a MitoSOX assay, and cytosol ROS was evaluated by a DCF assay. (b) Bar graph showing the averages of the red fluorescence (MitoSOX). (c) Bar graph showing the averages of the green fluorescence (DCF). Values represent the mean ± SEM (n = 10). ^∗∗p < 0.01, ^∗∗∗p < 0.001 vs. PrP.