Figure 5.

PrP (106-126) treatment upregulated calcineurin (CaN) activity. SK-N-SH cells were exposed to PrP (106-126) or scrambled PrP in a dose-dependent manner for 5 hours. (a) Cytosolic and nuclear fractions obtained from SK-N-SH cells induced for human calcineurin expression are analyzed by western blot with antibodies for CaN, for the cytosolic β-actin marker, and for the nuclear lamin A/C marker. (b) Bar graph representing the average nuclear CaN protein levels. The expression data were normalized to lamin A/C expression. The expression levels were evaluated by quantifying the protein bands, depicted by densitometric values beside the blot. Values represent the mean ± SEM (n = 5). ^∗∗p < 0.01, ^∗∗∗p < 0.001 vs. control.