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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Curr Eye Res. 2021 Mar 3;46(9):1271–1282. doi: 10.1080/02713683.2021.1884726

Table 1.

MTT Proliferation Assay

Time(h)Cell Human Control Human 1,25-VitD3 Human 24,25-VitD3 WT Mouse Control WT Mouse 1,25-VitD3 WT Mouse 24,25-VitD3 KO Mouse Control KO Mouse 1,25-VitD3 KO Mouse 24,25-VitD3
0 0.32±0.03 0.28±0.05 0.27±0.01 0.17±0.02 0.12±0.03 0.2±0.01 0.32±0.01 0.23±0.01 0.25±0.03
24 0.29±0.04 0.34±0.05 0.34±0.06 0.25±0.03 0.29±0.05 0.32±0.03 0.37±0.06 0.40±0.04 0.51±0.02
48 0.41±0.06 0.43±0.02 0.41±0.03 0.31±0.03 0.36±0.08 0.55±0.08 0.49±0.07 0.47±0.06 0.57±0.12
72 0.66±0.06 0.66±0.08 0.49±0.05 0.84±0.06 1.01±0.06 1.19±0.06 1.18±0.2 1.17±0.04 1.36±0.05
96 0.71±0.02 0.72±0.06 0.69±0.05 0.72±0.03 0.84±0.07 0.99±0.08 1.10±0.12 1.10±0.09 1.21±0.06

O.D. values at 540 nm of human and mouse corneal fibroblasts (WT and KO) cultured with 1,25-Vit D3 (20 nM) or 24,25-Vit D3 (100 nM). No significant differences found between groups examined.