Table 1.
MTT Proliferation Assay
| Time(h)╲Cell | Human Control | Human 1,25-VitD3 | Human 24,25-VitD3 | WT Mouse Control | WT Mouse 1,25-VitD3 | WT Mouse 24,25-VitD3 | KO Mouse Control | KO Mouse 1,25-VitD3 | KO Mouse 24,25-VitD3 |
|---|---|---|---|---|---|---|---|---|---|
| 0 | 0.32±0.03 | 0.28±0.05 | 0.27±0.01 | 0.17±0.02 | 0.12±0.03 | 0.2±0.01 | 0.32±0.01 | 0.23±0.01 | 0.25±0.03 |
| 24 | 0.29±0.04 | 0.34±0.05 | 0.34±0.06 | 0.25±0.03 | 0.29±0.05 | 0.32±0.03 | 0.37±0.06 | 0.40±0.04 | 0.51±0.02 |
| 48 | 0.41±0.06 | 0.43±0.02 | 0.41±0.03 | 0.31±0.03 | 0.36±0.08 | 0.55±0.08 | 0.49±0.07 | 0.47±0.06 | 0.57±0.12 |
| 72 | 0.66±0.06 | 0.66±0.08 | 0.49±0.05 | 0.84±0.06 | 1.01±0.06 | 1.19±0.06 | 1.18±0.2 | 1.17±0.04 | 1.36±0.05 |
| 96 | 0.71±0.02 | 0.72±0.06 | 0.69±0.05 | 0.72±0.03 | 0.84±0.07 | 0.99±0.08 | 1.10±0.12 | 1.10±0.09 | 1.21±0.06 |
O.D. values at 540 nm of human and mouse corneal fibroblasts (WT and KO) cultured with 1,25-Vit D3 (20 nM) or 24,25-Vit D3 (100 nM). No significant differences found between groups examined.