Skip to main content
. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Pregnancy Hypertens. 2021 May 14;25:39–47. doi: 10.1016/j.preghy.2021.05.002

Figure 2: Effect of TNF-α Blockade on Uterine Artery Resistance Index (UARI), Circulating Total Nitrate and 8-Isoprostane, and Placental cGMP and Reactive Oxygen Species (ROS).

Figure 2:

On Gestation Day (GD) 14, either the Reduced Uterine Perfusion Pressure (RUPP) procedure or a Sham procedure was performed on pregnant Sprague Dawley rats. On GD 15 and 18, vehicle or 0.4 mg/kg Etanercept (ETA) was injected i.p. in a subset of Sham and RUPP rats. On GD18, UARI (A) was measured via Doppler Ultrasound and on GD19, blood and placentas were collected under isoflurane anesthesia and frozen for analysis. Plasma was used to measure circulating total nitrate (B) and circulating 8-isoprostane (C) levels. Additionally, placental cGMP (D) and placental ROS (E) were measured. Sham: n=5–10; RUPP: n=5–10; Sham+ETA: n=5–10; RUPP+anti-IFNγ: n=5–10. All data are expressed as mean ± SEM. Statistical analyses were performed using two-way ANOVA with multiple comparisons followed by Tukey’s post-hoc test. *p<0.05 versus Sham; #p<0.05 vs RUPP.