Skip to main content
. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Pregnancy Hypertens. 2021 May 14;25:39–47. doi: 10.1016/j.preghy.2021.05.002

Figure 4: Effect of TNF-α Blockade on Circulating and Placental Cytokines.

Figure 4:

On Gestation Day (GD) 14, either the Reduced Uterine Perfusion Pressure (RUPP) procedure or a Sham procedure was performed on pregnant Sprague Dawley rats. On GD 15 and 18, vehicle or 0.4 mg/kg Etanercept was injected i.p. in a subset of Sham and RUPP rats. On GD19, blood and placentas were collected under isoflurane anesthesia and frozen for analysis of circulating TNF-α (A), circulating IL-6 (B), circulating IFNγ (C), placental TNF-α (D), placental IL-6 (E), and placental IFNγ (F). Sham: n=8–9; RUPP: n=8–10; Sham+ETA: n=7–9; RUPP+ETA: n=7–10. All data are expressed as mean ± SEM. Statistical analyses were performed using two-way ANOVA with multiple comparisons followed by Tukey’s post-hoc test. *p<0.05 versus Sham; #p<0.05 vs RUPP.