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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Pregnancy Hypertens. 2021 May 14;25:39–47. doi: 10.1016/j.preghy.2021.05.002

Figure 5: Western Blot analysis of placental eNOS expression.

Figure 5:

On Gestation Day (GD) 14, either the Reduced Uterine Perfusion Pressure (RUPP) procedure or a Sham procedure was performed on pregnant Sprague Dawley rats. On GD 15 and 18, vehicle or 0.4 mg/kg Etanercept was injected i.p. in a subset of Sham and RUPP rats. On GD19, placentas were collected following sacrifice and processed for analysis of placental eNOS expression. (A).Quantified fold change of placental eNOS expression normalized to β-actin. (B) A representative blot of eNOS and β-actin expression in all groups is shown. Sham: n=6; RUPP: n=6; Sham+ETA: n=6; RUPP+ETA: n=6. All data are expressed as mean ± SEM. Statistical analyses were performed using two-way ANOVA with multiple comparisons followed by Tukey’s post-hoc test. *p<0.05 versus Sham; #p<0.05 vs RUPP.