Skip to main content
. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: J Neurochem. 2021 Jul 7;158(3):737–752. doi: 10.1111/jnc.15455

Figure 3. Ischemia induced up-regulation of ganglioside GD3 and GD3S in the hippocampus occurred predominantly in microglia.

Figure 3

A. Representative DAB immunostaining images of anti-GD3 (brown) and counter stain of cresyl violet (purple) on brain sections from WT and GD3S-KO hippocampus at R2d. B. Representative IF image of GD3 and microglial marker CD11b on hippocampal brain sections from WT and GD3S-KO mice at R2d. C. IF staining of GD3 and astrocyte marker GLAST1 didn’t show significant co-localization. D. IF staining of GD3 and microglial marker CD11b showed localization of ganglioside GD3 in microglial cells in the injured hippocampal regions (CA1, CA3 and the hilus of dentate gyrus (DG)) after GCI. Zoomed area showed representative images of CA1. E. Quantification on the co-localization coefficiency of GD3 with CD11b showed a significant increase of the co-efficiency at 2 and 4 day after GCI (Sham n=4 mice/group, GCI n=6 mice/group). F. Quantification of the mean IF intensity of GD3 in the hippocampus (Sham n=4 mice/group, GCI n=5–6 mice/group). G and H. Western blot (G) and densitometry analysis (H) showed predominant up-regulation of GD3-synthase in lysate of CD11b+ microglia but not CD11b- cells. n=6 mouse/group, 2 mouse brains were pooled for isolating 1 sample of CD11b+/CD11b- cell lysates for Western blot. #p<0.05, ##p<0.01 compared to sham. One-way ANOVA followed by post-hoc. IF: Immunofluorescence. Scale bar: 20 μm.