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. Author manuscript; available in PMC: 2022 Aug 10.
Published in final edited form as: Immunity. 2021 Jun 8;54(8):1683–1697.e3. doi: 10.1016/j.immuni.2021.05.017

Fig 6: Acetate supplementation and Tip60 RNAi in Tk+ cells result in inverse patterns of 20E and IMD-regulated gene expression in the Drosophila intestine.

Fig 6:

RT-qPCR quantification of sad, shd, EcR, Eip75B, and Eip74F in the intestines of CV Tk>, Tk>Gcn5RNAi, and (A) Ada3RNAi (B) flies. RT-qPCR quantification of (C) PGRP-LC and (D) DptA in the intestines of CV Tk>, Tk>Gcn5RNAi, Tk>Ada3RNAi, Tk>Tip60RNAi, Tk>pontRNAi and Tk>EcRRNAi flies. RT-qPCR quantification of ecdysone-regulated genes in the intestines of CV Tk> and (E) Tk>EcRRNAi, (F) Tk>Tip60RNAi, and (G) Tk>pontRNAi flies. All experiments were performed in CV flies. The mean measurement is indicated. Error bars represent the standard deviation. Significance was assessed using a student’s t-test. ns not significant, * p<0.05, ** p<0.01, *** p<0.001, **** p<0.0001.