Figure 1. Panx1 expression restricts the severity of allergic airway inflammation.

(A) Relative Panx1 expression (log₂FC relative to healthy) on peripheral blood mononuclear cells (PBMC) of healthy or allergic asthmatic children (ctrl n=13, a.asthmatics n=14) (*p=0.015), revealing a decrease in Panx1 in asthmatics.

(B) Schematic representation of house dust mite (HDM)-induced allergic airway inflammation.

(C) H&E lung histology images of wild-type (n=13) and global Panx1^−/−(n=8) mice during PBS or HDM challenge. Arrows highlight areas of immune cell infiltration and inflammation. Disease severity score as assessed by a pathologist blinded to the genotypes (*p=0.05).

(D) Flow plots showing the extent of eosinophil and CD4⁺ T cell infiltration into the airways of Panx1^+/+ and Panx1^−/− mice after HDM-induced airway inflammation.

(E) Absolute cellularity of eosinophils (left) (*p=0.017), total CD4⁺ T cells (middle) (**p=0.008), and activated CD69⁺ CD4⁺ T cells (right) (*p=0.033). Each dot represents a mouse. (PBS control– Panx1^+/+ n=4, PBS control– Panx1^−/− n=5, HDM– Panx1^+/+ n=19, HDM– Panx1^−/− n=21) and lung (PBS– Panx1^+/+ n=3, PBS– Panx1^−/− n=3, HDM– Panx1^+/+ n=22, HDM– Panx1^−/− n=20), respectively, in wild-type (Panx1^+/+) and global Panx1^−/−.

(F) Flow plots showing the percentage of IL4+ CD4⁺ T cells (left) and quantification (right) during

HDM-induced allergic airway inflammation. (Panx1^+/+ n=4, Panx1^−/−n=6) (*p=0.011). Unpaired Student’s t-test (A,C,E,F). Related to Figure S1.