Table 1.
Lipidomics studies utilizing cadaveric or related biological samples
| Anthropogenic investigations | ||||||
|---|---|---|---|---|---|---|
| Research Focus | Source | Extraction Method | Internal Standard | Analytical Method | Instrument | Citations |
| Use of biomarkers in archaelogical context to define early manuring practice | Anthropogenic Soil (Scotland) | Bligh and Dyer | 5β-pregnan-3α-ol, hyocholic acid | High temperature-GC (HT-GC) | Hewlett-Packard 5890 Series II GC | Simpson et al. 1988 |
| investigate foodstuffs used in ceramic vessels by populations of the Indus Civilization in northwest India | Pottery fragments (Northwest India) | Acidified methanol, n-hexanes | Not specified | GC-MS, GC-C-IRMS | Agilent 7890 B GC, Agilent 5977 B MS; Hewlett Packard 7890B GC | Suryanaran et al.2020 |
| Faecal biomarkers for species identification | Faeces, soil | Solid phase extraction (ENVY disks) | 5α-cholestane | GC-MS | Shimadzu QP2010plus | Harrault et al. 2019 |
| Forensic/postmortem investigations | ||||||
| Membrane phospholipids as a biomarker for longer term PMI | Cadaveric Skeletal Muscle (quadriceps) | MTBE | ([2H31]PtdE 34:1, [2H54]PtdE 28:0, [2H31]PtdC 34:1, [2H54]PtdC 28:0) | ESI+MS/MS | Thermo Q Exactive | Langley et al. 2019 |
| Metabolites as biomarkers for PMI | Rat, Human muscle (biceps femoris) | chloroform/methanol/water solution 20:60:20 | Not specified | LC-MS | Thermo Q Exactive | Pesko et al. 2020 |
| Lipodomic analyses of postmortem tissues suitable to provide quantitative, objective PMI estimates | Cadaveric Skeletal Muscle (quadriceps) | MTBE | [2H8]arachidonic acid, [2H4]hexacosanoic acid, [2H31] PtdEtn 34:1, [2H31]PtdCh 34:1, and [2H31]PG 34:1 | ESI+MS/MS | Thermo Q Exactive | Wood et al. 2013 |
| Biomedical/Human health and disease investigations | ||||||
| Phospholipid profiles of human trabecular meshwork in glaucoma vs control | Cadaveric trabecular meshwork | Modified Bligh and Dyer | 1,2-ditridecanoyl-sn-glycero-3-phosphocholine, 1,2-dioleoyl-sn-glycero-3-phospho-L-serine, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine, 1,2-dioleoyl-sn-glycero-3-phospho-(10-myo-inositol) | LC-MS/MS | TSQ Quantum Access Max | Arbindi et al. 2013 |
| Lipidomics reveal impaired glucosylspingosine lipids pathway in glaucoma | Cadaveric optic nerve | Modified Bligh and Dyer | Not specified | LC-MS | Thermo Q Exactive | Chauhan et al. 2019 |
| Sphingolipid profile of diabetic cornea | Cadaveric cornea | Not specified | Not specified | LC-MS/MS | Shimadzu Nexera UPLC | Priyadarsini et al. 2015 |
| Lipid and protein profile in diabetic vitreous | Human vitreous | Acidified methanol, solid-phase extraction | 500 pg each of d4-PGE2, d8-12-HETE, d11-11(12)- DiHETrE (dihydroxyeicosatrienoic acid), d11-8(9)-EET, d6-20-HETE, and d8-11(12)-EET | LC-MS/MS | Q-trap 3200 | Schwartzman et al. 2010 |
| Sphingolipid and ceramide profiles of human trabecular meshwork | Cadaveric trabecular meshwork | Bligh and Dyer | N-oleoyl-D-erythro-sphingosylphosphorylcholine, D-er- ythro-sphingosine, D-erythro-sphingosine-1-phosphate, and N- oleoyl-D-erythro-sphingosine | GC-MS | TSQ Quantum Access Max | Aljohani et al. 2014 |
| Spingolipid profile in Small Ischemic Vascular Dementia (SIVD) | Cadaveric brain (temporal lobe) | Modified Bligh and Dyer | PC-14:0/14:0, PE-14:0/14:0, PS-14:0/14:0, PA-17:0/17:0, PG-14:0/14:0, PI-34:1/d31, LPC-C20:0, LPE-C17:0, C8-GluCer, C17-Cer, C14-LBPA, C12-SM, C12-SL | LC-MS/MS | Q-trap 3200 | Lam et al. 2013 |
| Donor liver biomarkers associated with early allograft dysfunction | Human Liver | Methanol | Not specified | LC-MS and MALDI | Xevo G2 | Li et al. 2017 |
| Aberrant lipid metabolism in Hepatocellular Varcinoma | Human Liver | Not specified | Not specified | LC-MS | Not specified | Cortes et al. 2014 |
| Changes in phospholipid profile in Alzheimer’s Disease | Cadaveric brain (prefrontal cortex) | MTBE | Not specified | MS | Xcalibur and nano me | Lefterov et al. 2014 |
| Loss of cardiac tetralinoleoyl cadiolipin in Heart Failure | Cadaveric heart (left ventricle) | Modified Bligh and Dyer | Not specified | not specified | Not specified | Sparagna et al. 2007 |
| APOE2 orchestrated differences in transcriptomic and lipidomic profiles in Alzheimer’s Disease | Cadaveric brain (parietal love) | Folch | PlsCho 16:0/18:1, 16:0/18:2, 16:0/20:4 and 16:0/22:6 | LC-MS/MS | Agilent 1290 | Otoki et al. 2021 |