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. 2021 Aug 13;12:4917. doi: 10.1038/s41467-021-25203-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a p65 localization in MCF10A cells after HU + ATRi treatment. Scale bar: 10 μm. b Quantification of nuclear p65 in MCF10A cells treated with HU + ATRi for 32 h. Mean values ± SD (n = 3). **P < 0.01 (two-tailed Welch t-test). c MCF10A cells were treated with HU and/or ATRi for 32 h and the levels of IkBα, p65, Chk1-pS345, and γH2AX were analyzed by western blotting. d p65 ChIP was performed in MCF10A cells treated with HU + ATRi. p65 binding at A3A promoter was determined by qPCR. The results are representative of three independent experiments and qPCR was done in triplicate. Mean values ± SD. ****P < 0.0001 (two-tailed Welch t-test). e A3A mRNA level quantification after HU + ATRi for 32 h in BICR6 WT or knockout for p65. Mean values ± SD (n = 3). ***P < 0.001 (two-tailed Welch t-test). f BICR6 cells were transfected with CTL or p65 siRNA for 40 h following by HU + ATRi treatment for 32 h. A3A expression level was determined by RT-qPCR. Mean values ± SD (n = 3). **P < 0.01 (two-tailed Welch t-test). g Heat maps of inflammatory genes expression level in BICR6 WT or p65 KO treated with HU + ATRi. h BICR6 cells were transfected with CTL or p65 siRNA for 18 h following by IR + ATRi treatment for 72 h. A3A expression level was determined by RT-qPCR. Mean values ± SD (n = 3). ****P < 0.0001 (two-tailed Welch t-test). i The A3A mRNA was quantified in unchallenged BICR6 and MCF10A cells. Mean values ± SD (n = 3). **P < 0.01 (two-tailed Welch t-test). j Quantification of nuclear p65 in the BICR6 and MCF10A cells in unchallenged conditions. Mean values ± SD (n = 3). ****P < 0.0001 (two-tailed Welch t-test). k The A3A mRNA was quantified in the indicated cell lines in unchallenged conditions. Mean values ± SD (n = 3). ***P < 0.001 (two-tailed Welch t-test). i BICR6 cells were transfected with indicated siRNA for 40 h followed by HU + ATRi treatment for 32 h. A3A expression level was determined by RT-qPCR. Mean values ± SD (n = 3). **P < 0.01 (two-tailed Welch t-test). Source data are provided as a Source Data file.