Fig. 1. LATS2 is activated under diabetogenic conditions.

a Schematic structure and mechanism of action for LATS-BS. b–e INS-1E cells transfected with the firefly luciferase reporter plasmids N-luc-YAP15-S127, C-luc-14-3-3, and pRL-Renilla luciferase were treated with 22.2 mM glucose alone or in combination with 0.5 mM palmitate for 24 h. b, c Downstream phosphorylation of the exogenously expressed YAP-S127 fragment was determined by firefly luciferase activity and normalized to the Renilla signal (n = 3 biologically independent samples). d, e Representative western blots and pooled quantitative densitometry analysis for exogenously expressed YAP-127 by a phospho-specific antibody are shown (n = 6 biologically independent samples). Isolated islets from f HFD-treated C57BL/6 J mice for 16 weeks or g the obese diabetic leptin receptor-deficient db/db mice and their corresponding controls cultured overnight and transfected with the N-luc-YAP15-S127 plasmid for 24 h. Representative western blots and pooled quantitative densitometry are shown (n = 3 independent experiments) and results were normalized to the respective control conditions and ratios, in which a normal distribution of results cannot be proven, were analyzed. Data are expressed as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001; all by two-tailed Student’s t-tests.