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. 2021 Aug 13;12:4928. doi: 10.1038/s41467-021-25145-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a–j β-LATS2^−/− mice (n = 14), RIP-Cre (n = 7) and LATS2^fl/fl controls (n = 10) injected with streptozotocin (STZ) (40 mg per kg body weight for 5 consecutive days) or saline (β-LATS2^−/− n = 4; LATS2^fl/fl n = 5) for 5 consecutive days. a Random fed blood glucose measurements after first saline or STZ injection (day 0) over 21 days and b i.p. glucose tolerance test (GTT) at day 19 in β-LATS2^−/−, Rip-Cre and LATS2^fl/fl mice. c Insulin secretion during an i.p.GTT measured before (0 min) and 15 min after glucose injection and expressed d as ratio of secreted insulin at 15 min to that secreted at 0 min (stimulatory index) (Rip-Cre STZ n = 6; LATS2^fl/fl STZ n = 8; β-LATS2^−/− STZ n = 11). e Ratio of secreted insulin and glucose calculated at fed state (Rip-Cre STZ n = 7; LATS2^fl/fl STZ n = 9; β-LATS2^−/− STZ n = 12). f–j Mice were sacrificed at day 22. f β-cell mass (given as percentage of the whole pancreatic section from 10 sections spanning the width of the pancreas) (Rip-Cre STZ n = 4; LATS2^fl/fl STZ n = 5; β-LATS2^−/− STZ n = 8). Representative images and quantitative analyses from triple staining for Ki67 (g, h) or TUNEL (i, j), insulin and DAPI expressed as percentage of TUNEL- or Ki67-positive β-cells ±SEM (Ki67: Rip-Cre STZ n = 7; LATS2^fl/fl STZ n = 10; β-LATS2^−/− STZ n = 14; TUNEL: Rip-Cre STZ n = 7; LATS2^fl/fl STZ n = 10; β-LATS2^−/− STZ n = 12). Data are expressed as means ± SEM. ⁺p < 0.001 LATS2^fl/fl-STZ or RIP-Cre-STZ to LATS2^fl/fl control mice. ⁺⁺p < 0.01 LATS2^fl/fl-STZ or RIP-Cre-STZ to LATS2^fl/fl control mice. ⁺⁺⁺p < 0.05 LATS2^fl/fl-STZ or RIP-Cre-STZ to LATS2^fl/fl control mice. *p < 0.05 β-LATS2^−/−-STZ to LATS2^fl/fl-STZ or RIP-Cre-STZ. **p < 0.01 β-LATS2^−/−-STZ to LATS2^fl/fl-STZ or RIP-Cre-STZ. ***p < 0.001 β-LATS2^−/−-STZ to LATS2^fl/fl-STZ or RIP-Cre-STZ. ^§p < 0.05. One-way ANOVA with Tukey’s post hoc test for (a, b, d, e, h, j); by two-tailed Student’s t-tests for (c, f). scale bar depicts 10 μm.