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. 2021 Aug 13;12:4928. doi: 10.1038/s41467-021-25145-x

Fig. 7. LATS2 induces β-cell apoptosis by activating mTORC1 pathway.

Fig. 7

a, b Representative western blots and pooled quantitative densitometry analysis (lower panels) of INS-1E cells (a) and human islets (b) transduced with LacZ control or LATS2 adenoviruses for 48 h (n = 4 and 3 independent experiments respectively for a, b). c Representative western blot and pooled quantitative densitometry analysis (lower panel) of INS-1E cells transfected with LATS2 siRNA or control siScr and treated with the 22.2 mM glucose (n = 3 independent experiments). d Representative western blot and pooled quantitative densitometry analysis (lower panel) of human islets transduced with Ad-hShLATS2 and treated with 22.2 mM glucose plus 0.5 mM palmitate for 72 h (n = 3 different human islets isolations). e, f Representative western blots and pooled quantitative densitometry analysis (lower panels) of INS-1E cells (e) and human islets (f) transduced with LacZ control or LATS2 adenoviruses for 24 h and then exposed to 100 nM Rapamycin or 10 μM S6K1 inhibitor (S6K1i) for additional 24 h (n = 4 and 3 independent experiments respectively for (e, f); n = 2 for clPARP). g Representative western blot and pooled quantitative densitometry analysis (lower panel) of INS-1E cells transfected with siRaptor or siScr and then transduced with Ad-LacZ or Ad-LATS2 for 48 h (n = 3 independent experiments). Data are expressed as means ± SEM. Pooled quantitative densitometry of western blots were normalized to the respective control conditions and ratios (except b, f), in which a normal distribution of results cannot be proven, were analyzed. *p < 0.05, **p < 0.01, ***p < 0.001; all by two-tailed Student’s t-tests.