Fig. 1. Constitutive perinuclear tethering of rDNA is detrimental.

a Scheme of synthetic rDNA tethering through the expression of GFP- and GBP-fusion proteins. Lrs4 binds ribosomal repeats through interaction with the rDNA-bound proteins Net1 or Tof2. G, GFP. b Fivefold serial dilutions of WT, ^GFPLrs4, Net1^GFP, and Tof2^GFP cells transformed with empty vector or a plasmid bearing HEH1 fused to GFP-binding protein (GBP). c Fivefold serial dilutions of WT cells transformed with empty vector or plasmids bearing the indicated GFP-tagged fusion proteins. b, c Cells were spotted and grown on selective media with 2% glucose (control, Glc) or 2% galactose (induction, Gal) at 30 °C for 3 days. All constructs contain the galactose-inducible GAL1 promoter.