Fig. 4. SUMO overexpression promotes CLIP-cohibin disassembly and rDNA release and recombination.

a Co-immunoprecipitation of Nur13HA with Heh1^GFP or ^GFPLrs4 in cells expressing SUMO at endogenous levels or overexpressed from the ADH1 promoter (^6HisSUMO-OE). Dpm1 served as loading control. b Rates of rDNA recombination in WT (n = 4) or lrs4∆ (n = 3) cells expressing SUMO at endogenous levels or overexpressed from vectors with the GAL1 promoter (SUMO-OE), grown in galactose-containing media for 4 days. Data are mean ± SEM of n independent biological replicates, shown in log₂ scale relative to WT. Marker loss is calculated as in Fig. 3. Statistical analysis was performed using ANOVA, and letters denote significant differences with a Tukey’s post hoc test at P ≤ 0.05. c Percentage of undamaged cells with endogenous (WT) or overexpressed (SUMO-OE) SUMO levels with rDNA repeats localized outside the nucleolus, monitored as described for Fig. 2. Quantification of the marked rDNA unit was scored from four independent biological replicates, and the mean ± SEM is shown. Statistical analysis was performed using two-tailed Student’s t-test. Source data are provided as a Source Data file.