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. 2021 Aug 13;12:4910. doi: 10.1038/s41467-021-24879-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a The expressed Flag-fused siRNA-resistant (siR) proteins and endogenous SPF45 in HeLa cells were checked by Western blotting. Displayed blots are representative of three independent experiments. Arrowheads indicate degraded siRNA-resistant (siR) proteins, but not endogenous SPF45 (see ‘vector’ lane for the depletion efficiency of endogenous SPF45). b After the co-transfection of the indicated siRNA-resistant plasmids and three mini-gene plasmids, splicing efficiencies of the indicated three mini genes were analyzed by RT-PCR. Means ± SEM are given for three independent experiments and two-tailed Student t-test values were calculated (HNRNPH1 intron: p = 0.0175 for Control vs Vector, p = 0.8177 for Control vs Flag-SPF45/siR, p = 0.0270 for Control vs Flag-SPF45/UHMmt/siR, p = 0.0250 for Control vs Flag-SPF45/ΔG/siR; EML3 intron: p = 0.0012 for Control vs Vector, p = 0.1085 for Control vs Flag-SPF45/siR, p = 0.0035 for Control vs Flag-SPF45/UHMmt/siR, p = 0.0057 for Control vs Flag-SPF45/ΔG/siR; MUS81 intron: p = 0.0020 for Control vs Vector, p = 0.1693 for Control vs Flag-SPF45/siR, p = 0.0177 for Control vs Flag-SPF45/UHMmt/siR, p = 0.0004 for Control vs Flag-SPF45/ΔG/siR). *P < 0.05, **P < 0.01, ***P < 0.001, ^n.s.P > 0.05. Source data of all the above panels are provided as a Source Data file.