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. 2021 Aug 13;11:16487. doi: 10.1038/s41598-021-95823-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Unperturbed viral replication by IRF7 in corneal endothelial cells. (a) The level of HSV-1 DNA in HCEn cells was determined at 24 h after the infection using real-time PCR. The amount of HSV-1 DNA is not reduced for IRF7-competent wild type HCEn cells. N = 6. (b) Transcript of an immediately early gene infected cell protein 0 (ICP0) of HSV-1, was evaluated in HCEn cells at 12 h after infection using real-time RT-PCR. ICP-0 induction is not perturbed for IRF7-competent wild type HCEn cells. N = 8. (c) Titration plaque assay for HCEn cells after infection. The HSV-1 titer is not perturbed for IRF7-competent wild type HCEn cells. N = 6.