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. 2021 Aug 13;12:4922. doi: 10.1038/s41467-021-25190-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — aRRM1/SNP sequencing after subclonal analysis of HEK293T at D4. C/C/G genotype in RRM1 (in gray) on two SNP positions for NT cells. After editing, clones presented SNP-LOH with C/C (in pink) or C/G genotype (in red). Frequencies of SNP losses are indicated in pie chart for each protocol. LOH frequencies with protocols 1–3 were compared to non-transduced cells by two-sided Chi-square tests. Indels frequency was 63, 83 56% for protocols 1–3, respectively, n correspond to analyzed cells from each polyclonal pool (1 per protocol). b Chromosome analysis in HEK293T clones depending on SNP screening. Left, DNA-FISH (Scale-bar: 5 µm) and array-CGH of one LOH-negative clone. Right, DNA-FISH and array-CGH of LOH-positive clones from protocols #1–3. Deleted area is in red. n correspond to analyzed SNP loss cells (from protocols #1–3). Source data are provided as a Source Data file.