KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
| h5MP1 and its 7A and BN1 mutant forms | This paper | N/A |
| GST-h5MP1 and its 7A and BN1 mutant forms | This paper | N/A |
| GB-heIF3c20-102 | This paper | N/A |
| GB- heIF2β53-136 | This paper | N/A |
| Critical commercial assays | ||
| Dual-Glo® Luciferase Assay System | Promega | E2940 |
| Nano-Glo® Luciferase Assay System | Promega | N1110 |
| Deposited data | ||
| Raw MS data | This paper (Fig. 4A–C) | JPST001188 |
| Homology model structure of h5MP1 and its docking simulations | This paper (Fig. 4D&S7) | https://github.com/10ackert/5MP-PIC |
| Experimental models: Cell lines | ||
| Human embryonic kidney (HEK) 293T | John A. Chiorini | N/A |
| D. melanogaster cell line S2 | Erika Geisbrecht | N/A |
| Experimental models: Organisms/strains | ||
| S. cerevisiae S288C: his4-301(ACG) trp1 leu1 ura3 sui1Δ p(sui1-mof2-1 TRP1) | (Tang et al., 2017) | Asano lab KAY1027 |
| S. cerevisiae S288C: his4-301(ACG) trp1 leu1 ura3 sui1Δ p(sui1- K60E TRP1) | This paper | Asano lab KAY1121 |
| D. melanogaster: GMR-Gal4, (CGG)90-eGFP BD/Cyo | (Jin et al., 2003; Todd et al., 2010) | Todd Lab #34 |
| D. melanogaster: UAS-CGG90-eGFP-BD/CyO; Tub5/TM3-Ser line B | (Todd et al., 2010) | Todd Lab #503 |
| D. melanogaster: y[1] w[1118]; P{w[+mC]=lacW}kra[j9B6]/TM3, Sb[1] | Bloomington Drosophila Stock Center |
10216 |
| D. melanogaster: y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF02556}attP2 | Bloomington Drosophila Stock Center |
27248 |
| D. melanogaster: y[1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.GL00690}attP2 | Bloomington Drosophila Stock Center |
38918 |
| D. melanogaster: UAS-d5MP/Cyo | BestGene, this paper | 28098-1-M3-M-Ch2, Todd Lab #1218 |
| D. melanogaster: UAS-d5MP/Cyo | BestGene, this paper | 28098-1-M4-M-Ch2, Todd Lab #1219 |
| D. melanogaster: UAS-d5MP/Cyo | BestGene, this paper | 28098-1-M5-M-Ch2, Todd Lab # 1220 |
| Oligonucleotides | ||
| gcGGATCCGAATTCatgtcgcggtttttcaccacc | This paper (p1834 & p1835) | Asano lab 3c0_N |
| gcGGATCCGAATTCgaggagctcgtcaccaaac | This paper (p1836 & p1837) | Asano lab 3c1_N |
| ccGGATCCctcgctcagcaacaatgg | This paper (p1834 & p1836) | Asano lab 3c1_C |
| ggGGATCCgacaccttctttgtccacaatg | This paper (p1835 & p1837) | Asano lab 3c2_C |
| gcaatactgcaatggtatcaggaagcacaggttgctcaaggccaa agtgtttttcttgac |
This paper (p1659 & p1818) | Asano lab h5MP1-BN1/S |
| aaaaacactttggccttgagcaacctgtgcttcctgataccattgcagtattgcttcttc | This paper (p1659 & p1818) | Asano lab h5MP1-BN1/AS |
| ggagatcatcttgcagtggtaccaggagggccagtcaaaccagggccaaatgcatttcc | This paper (p1964) | Asano lab Dme5MP-BN1/S |
| ggaaatgcatttggccctggtttgactggccctcctggtaccactgcaagatgatctcc | This paper (p1964) | Asano lab Dme5MP-BN1/AS |
| GtagcggcgagcgcgggcggcggcggtgacggagG | This paper (p1785) | Asano lab hFMR1_1/S |
| GATCCctccgtcaccgccgccgcccgcgctcgccgctaCTG CA |
This paper (p1785) | Asano lab hFMR1_1/AS |
| Recombinant DNA | ||
| pGEX-h5MP1; GST-fusion to h5MP1 | (Singh et al., 2011), (Fig. 1 & S1) | Asano lab p1477 |
| pGEX-h5MP1-7A; pGEX-h5MP1 carrying 7A | (Singh et al., 2011), (Fig. 1 & S1) | Asano lab p1116 |
| pGEX-h5MP1-BN1; pGEX-h5MP1 carrying BN1 | This paper (Fig. 1&S1) | Asano lab p1818 |
| pGB-heIF2β-K2K3; GB1-fusion to heIF2β53-136 | (Luna et al., 2012) (Fig. 1&S1) | Asano lab p1336 |
| pGB-heIF3c12-His; GB1-fusion to heIF3c20-102 | This paper (Fig. 1&S1) | Asano lab p1837 |
| pGB-heIF3c01-His; GB1-fusion to heIF3c1-40 | This paper (Fig. S1) | Asano lab p1834 |
| pGB-heIF3c02-His; GB1-fusion to heIF3c1-102 | This paper (Fig. S1) | Asano lab p1835 |
| pGB-heIF3c1-His; GB1-fusion to heIF3c20-40 | This paper (Fig. S1) | Asano lab p1836 |
| pEF1A-h5MP1; 3xF-h5MP1 under the eEF1A promoter | (Kozel et al., 2016) (Fig. 1, 3–5) | Asano lab p1556 |
| pEF1A-h5MP1-BN1; pEF1A-h5MP1carrying BN1 |
This paper (Fig. 1, 3–5) | Asano lab p1659 |
| pEF1A-h5MP1-7A; pEF1A-h5MP1carrying 7A | (Kozel et al., 2016) (Fig. 3–5) | Asano lab p1667 |
| pEF1A-h5MP2; 3xF-h5MP2 under the eEF1A promoter | (Kozel et al., 2016) (Fig. 3–5) | Asano lab p1660 |
| pEF1A-heIF5; 3xF-heIF5 under the eEF1A promoter | (Kozel et al., 2016) (Fig. 3–5) | Asano lab p1558 |
| YEpL-TIF5-F; hc TIF5-F LEU2 plasmid | (Asano et al., 1999) (Fig. S2) | Asano lab p313 |
| YEpL-GB-TIF5B5-F; hc LEU2 plasmid encoding GB1-fusion to yeIF5201-405-F under TIF5 promoter | This paper (Fig. 2 & S2) | Asano lab p1206 |
| YEpL-GB-TIF5B5 -BN1-F; YEpL-GB-TIF5B5-F carrying BN1 | This paper (Fig. 2 & S2) | Asano lab p1263 |
| YEpL-h5MP1-F; h5MP1-F LEU2 plasmid | (Tang et al., 2017) (Fig. 2 & S2) | Asano lab p1587 |
| YEpL-h5MP1-7A-F; hc h5MP1-7A-F LEU2 plasmid | Tang et al., 2017) (Fig. 2 & S2) | Asano lab p1621 |
| pCDNA-h5MP1; h5MP1 under the CMV promoter | (Singh et al., 2011) (Fig. S5) | Asano lab p1910 |
| pAC-Dme5MP; Drosophila Kra under the fly actin promoter | (Kozel et al., 2016) (Fig. 4) | Asano lab p1708 |
| pAC-Dme5MP-BN1; pAc-Dme5MP carrying BN1 |
This paper (Fig. 4) | Asano lab p1964 |
| pLKO-sh5MP1 0140053 (# 2) | (Kozel et al., 2016) (Fig. 3 & 5) | Asano lab p1462 |
| pLKO-sh5MP1 0139465 (# 5) | (Kozel et al., 2016) (Fig. 3 & 5) | Asano lab p1465 |
| pLKO-sh5MP2 0147346 (# 7) | (Kozel et al., 2016) (Fig. 3 & 5) | Asano lab p1467 |
| pLKO-sh5MP2 0148768 (# 9) | (Kozel et al., 2016) (Fig. 3 & 5) | Asano lab p1469 |
| pLKO-sh5MP2 0147812 (# 10) | (Kozel et al., 2016) (Fig. 3 & 5) | Asano lab p1470 |
| pLKO-shGFP | (Kozel et al., 2016) (Fig. 3 & 5) | Asano lab p1471 |
| pSV40-firefly Kozak AUG; AUG under a typical Kozak context | (Ivanov et al., 2010) (Fig. 3) | Asano lab p1523 |
| pSV40-firefly Kozak CUG; CUG under a typical Kozak context | (Ivanov et al., 2010) (Fig. 3) | Asano lab p1521 |
| pSV40-firefly Kozak GUG; GUG under a typical Kozak context | (Ivanov et al., 2010) (Fig. 3) | Asano lab p1520 |
| pSV40-firefly Kozak ACG; ACG under a typical Kozak context | (Ivanov et al., 2010) (Fig. 3) | Asano lab p1525 |
| pSV40-Renilla Kozak AUG; Renilla control plasmid: AUG under a typical Kozak context | (Ivanov et al., 2010) (Fig. 3) | Asano lab p1526 |
| pSV40 firefly FMR1_24; ACG under FMR1_24 context | This paper (Fig. 5E) | Asano lab p1785 |
| AUG-nLuc -3xF; AUG-Nluc control plasmid | (Kearse et al., 2016) (Fig. 5 & S5) | Asano lab p1810 |
| GGG-nLuc -3xF; GGG-Nluc control plasmid | (Kearse et al., 2016) (Fig. 5 & S5) | Asano lab p1811 |
| +1 (CGG)100-nLuc -3xF; +1 (CGG)100-nLuc -3xF | (Kearse et al., 2016) (Fig. 5 & S5) | Asano lab p1812 |
| +2 (CGG)100-nLuc -3xF; +2 (CGG)100-nLuc -3xF | (Kearse et al., 2016) (Fig. 5 & S5) | Asano lab p1813 |
| pGW 5mp-2A-mapple #10 | This paper (Fig. S6B) | Todd Lab #BG109 |
| pGW 5mp-7a-2A-mapple #10 | This paper (Fig. S6B) | Todd Lab #BG111 |
| pGW 5MP-BN1-2a-mApple | This paper (Fig. S6B) | Todd Lab #BG284 |