Table I.
Selected Sample Preparation Parameters of Urinary PHMA Methods
| Method | |||||
|---|---|---|---|---|---|
| Parameter | A | B | C | D | E |
| Sample preparation | Dilute and shoot | Dilute and shoot | SPE | SPE | Liquid/liquid extraction |
| Sample treatment pHa | 6.8 | 2.9 | 1.4 | ∼0.5 | ∼4.5 |
| Treatment time (min) | – | – | No treatment time | 10 | 10 |
| Acid used for sample pretreatment | – | – | 4% H3PO4 | 36% HCl | KH2PO4 |
| Sample diluent | 15 mM NH4OAc | 5 mM NH4HCO2, 0.15% HCOOH | – | – | – |
| Reconstitution solvent | – | – | 0.05% CH3COOH | 30% methanol in 15 mM NH4OAc | Methanol |
a
Used diluent pH for dilute-and-shoot methods. “–” is not applicable.