Figure 4.

Anesthesia and preprocedural preparations and set-up. Laser protection goggles must remain on all assistants while the laser is operational. The tetracaine drop, anesthetics (ketamine/xylazine), tropicamide, and the sterile hypromellose ophthalmic demulcent solution are filtered, aliquoted, labeled, and secured on a rack (red arrows). The anesthesia cocktail is prepared and drawn in an insulin syringe (red arrowhead). After intraperitoneal injection of anesthetic medications, the FA confirms that the animal is not responsive by checking the response to stimuli, then proceeds to trim the whiskers with surgical scissors.