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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Lab Invest. 2021 Jan 6;101(5):661–676. doi: 10.1038/s41374-020-00518-w

Figure 2. 3D multiphoton microscopy of unlabeled nephrectomy.

Figure 2.

Mosaic of high-resolution image volumes collected from a 4 mm by 9 mm, 50 micron thick section of paraformaldehyde-fixed human nephrectomy tissue. Panel A – Maximum projection image of 3D volume of tissue autofluorescence. Panel B- Maximum projection of 3D volume of second harmonic generation (SHG) images. Panel C – Overlay of autofluorescence and SHG. Panels D-F show corresponding 4X magnification images of the region indicated in the upper box in panel A, and panels G-I show corresponding 4X magnification images of the region indicated in the lower box in panel A. Arrows in panels G and H indicate regions of apparent tubular dropout and fibrosis. Scale bar in panel A represents 1 mm. Scale bar in panel D represents 250 microns in D-I.