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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Lab Invest. 2021 Jan 6;101(5):661–676. doi: 10.1038/s41374-020-00518-w

Figure 3. 3D confocal immunofluorescence of structural markers.

Figure 3.

Panel A - Maximum projection of combined autofluorescence and SHG images. Panel B - Maximum projection of mosaic of confocal fluorescence image volumes of Oregon-Green phalloidin. Panel C - Overlay of maximum projection confocal fluorescence images of phalloidin (green) and antibodies to Tamm-Horsfall Protein (THP, cyan) and aquaporin-1 (AQP1, magenta). Panels D-F show corresponding 4X magnification images of the region indicated in the upper box in panel A, and panels G-I show corresponding 4X magnification images of the region indicated in the lower box in panel A. Arrows in panels G-I indicate regions of fibrosis and apparent tubular dropout. Scale bar in panel A represents 1 mm. Scale bar in panel D represents 250 microns.