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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Lab Invest. 2021 Jan 6;101(5):661–676. doi: 10.1038/s41374-020-00518-w

Figure 5. 3D confocal fluorescence and multiphoton autofluorescence/SHG microscopy of regions of apparent injury.

Figure 5.

Panel A - Maximum projection of combined 3D fluorescence image volume of phalloidin (green) and antibodies to THP (cyan), AQP1 (magenta), myeloperoxidase (MPO, red), CD68 (yellow) and CD3 (white). Panels B-E - 4X magnifications of regions indicated in boxes in panel A. Top row – confocal fluorescence images. Middle row – corresponding autofluorescence images. Bottom row – corresponding SHG images. Scale bar in panel A represents 1 mm and in panel B 250 um for B-E.