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. 2021 Jul 13;53:101296. doi: 10.1016/j.molmet.2021.101296

Figure 4.

Figure 4

Up-regulation of hepatic RAMP2 in lean adult male mice is not associated with a phenotypic change. A: Mouse body weight after 18 days upregulation of hepatic mouse RAMP2 (AAV-alb-RAMP2) or control (AAV-alb-GFP); n = 4; data are mean ± SEM. B: Glucose tolerance test after 5-hour fast. C: Glucose tolerance test after 24-hour fast. D: Insulin tolerance test. E: Glucagon challenge. B–E experiments performed in mice 3–4 weeks post-AAV injection; n = 9–10 per group; data are mean ± SEM, with AUC shown as inset; unpaired t-test; ∗p < 0.05. F: cAMP dose response to GCG in isolated primary hepatocytes from AAV-alb-GFP and AAV-alb-RAMP2 mice, normalised to forskolin (10 μM) responses; n = 4; 4-parameter fit of pooled data shown; n = 7 separate mice from each cohort harvested over 4 days.