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. 2021 Jul 21;53:101305. doi: 10.1016/j.molmet.2021.101305

Figure 4.

Figure 4

BAT Rab10 is not required to maintain body weight temperature when exposed to severe cold. A-D. Rectal temperature in female (A&B) and male (C&D) mice under acute (A&C) or chronic (B&D) cold (4 °C) (n = 7 mice/group). E-H. Bodyweight overtime (ad libitum) (E&G) and adipose tissue depots weights (F&H): epididymal adipose tissue (eWAT), inguinal adipose tissue (iWAT), and brown adipose tissue (BAT) of female (E&F) and male (G&H) mice after 14 days at 4 °C (n = 7 mice/group). I. mRNA expression for UCP1, PRDM16, DIO, and CIDEA in BAT samples of female mice housed at 22 °C (n = 4–6 mice/group) or after 14 days at 4 °C (n = 7 mice/group). J. UCP1, Rab10 representative immunoblots of BAT extracts from control and bR10KO mice housed at 4 °C. K&L. Representative H and E staining images of BAT (K.) and iWAT (L.) of mice housed at room temperature (22 °C) and chronic cold (4 °C) conditions. Images are shown at 20X magnification with 60 μm scale bars. M. mRNA expression for UCP1, PRDM16, DIO2, and CIDEA in iWAT samples of female mice housed at 22 °C (n = 6 mice/group) or after 14 days at 4 °C (n = 7 mice/group). Unpaired Student's t-test was used for comparing groups. ∗p < 0.05 and ∗∗∗p < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)