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. 2021 Jul 21;53:101305. doi: 10.1016/j.molmet.2021.101305

Figure 6.

Figure 6

ChREBPβ and DNL-related gene expression are blunted in Rab10KO BAT and eWAT in a cell-intrinsic manner. A. mRNA expression for ChREBPβ, Fasn, and ACC1in BAT samples from female mice aged 9–12 weeks at thermoneutrality (30 °C) for 3 weeks (n = 6–7 mice/group). B. Representative immunoblots of brown adipose tissue (BAT) extracts from control and bR10KO mice at 30 °C. C. mRNA expression of ChREBPα and Srebp1c in BAT samples from female mice aged 9–12 weeks at thermoneutrality (30 °C) for 3 weeks (n = 6–7 mice/group). D. mRNA expression for ChREBPβ, Fasn, ACC1, ChREBPα, and Srebp1c in BAT samples from female mice aged 9–12 weeks at room temperature (n = 5–6 mice/group). E. mRNA expression for key fatty acid transportation proteins and fatty acid lipolysis and β-oxidation enzymes in BAT samples from female mice aged 9–12 weeks acclimated to thermoneutrality (30 °C) (n = 6–7 mice/group). F&G. mRNA expression for ChREBPβ, Fasn, ACC1, ChREBPα, and Srebp1c in eWAT samples from 12 weeks of age control and bR10KO females (F.) and from 12 weeks of age control and aR10KO females (G.). Unpaired Student's t-test was used for comparing groups. ∗p ≤ 0.05, ∗∗p < 0.01,∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article).