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. 2021 Aug 14;52:108. doi: 10.1186/s13567-021-00976-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Scheme for isolation of crypts from equine jejunum and subsequent 3D culture.A Section of equine jejunum. B Recovered villi (open arrows) and crypts (closed arrows). C Spheroid after four days of culture. D Enteroid after seven days of culture. E Enteroid at four days of culture after the third passage. Representative results from one of three horses. For details see Material and Methods (Photo: Nikon Coolpix 990).